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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in several tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was observed to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital role in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be described. Two more splice variants have been reported but in-depth nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind precisely towards the concentrate on antibody and block antibody binding. These peptide generally includes the epitope acknowledged with the antibody.
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This guidebook outlines further more doable optimization of cellular and immunoassay parameters to ensure the absolute best outcomes are...
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This guideline outlines further more attainable optimization of mobile and immunoassay parameters to be certain the best possible effects are...
Antibodies certain to the blocking peptide not bind on the epitope within the focus on protein. By comparing the staining from the blocked antibody compared to the antibody by itself, you can see which staining is unique.
Target Details Cyclin E belongs for the really conserved cyclin family, whose associates are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins show unique expression and degradation designs which contribute to the temporal coordination of each mitotic party. Cyclin E varieties a posh with and capabilities to be a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S changeover. This protein accumulates within the G1-S section boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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